Composite

Part:BBa_K3040507:Design

Designed by: TIEN , TING-LUN   Group: iGEM19_NTHU_Taiwan   (2019-10-15)


pFadBA_NTHU mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 670
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To express lipase on the outer cell membrane, we modified and improved the submitted Lpp-OmpA by iGEM15_NCTU-Formosa (BBa_K1694002 https://parts.igem.org/Part:BBa_K1694002). In our design, we replace the original NcoI restriction site with restriction sites AvrII and NheI which brings some benefits to the future application of Lpp-OmpA. First, we found that NcoI is a restriction site on the plasmid, pSB1C3, a commonly used plasmid in iGEM. Therefore, with this modification, Lpp-OmpA is able to apply to common used plasmid in iGEM. Furthermore, with the two designed restriction sites, we can link various protein with Lpp-OmpA simply by adding AvrII and NheI restriction sites in front and behind the gene sequence.


Source

P

References